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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

14,630 Views

09:40 min

September 28, 2018

DOI:

09:40 min
September 28, 2018

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Summary

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Here, we present a protocol for co-immunoprecipitation and an on-bead enzymatic activity assay to simultaneously study the contribution of specific protein domains of plasma membrane receptors to both enzyme recruitment and enzyme activity.

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