Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Michael Peled; Marianne Strazza; Adam Mor

doi:10.3791/58433

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免疫学と感染

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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

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JoVE Journal 免疫学と感染

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

14,630 Views

•

09:40 min

•

September 28, 2018

DOI:

10.3791/58433-v

DOI:

10.3791/58433-v

•

09:40 min

September 28, 2018

•

21 Views

Michael Peled¹, Marianne Strazza², Adam Mor²

¹Pulmonary Department,The Chaim Sheba Medical Center, ²Columbia Center for Translational Immunology, Department of Medicine,Columbia University Medical Center

概要

Here, we present a protocol for co-immunoprecipitation and an on-bead enzymatic activity assay to simultaneously study the contribution of specific protein domains of plasma membrane receptors to both enzyme recruitment and enzyme activity.

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Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

概要

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