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Culturing and Manipulation of O9-1 Neural Crest Cells
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Genetics
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JoVE Journal Genetics
Culturing and Manipulation of O9-1 Neural Crest Cells

Culturing and Manipulation of O9-1 Neural Crest Cells

English

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9,435 Views

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08:32 min

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October 09, 2018

DOI:

10.3791/58346-v

DOI:
10.3791/58346-v

•

08:32 min
October 09, 2018

•

5 Views
, , ,
1Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern California

Summary

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O9-1 is a multipotent mouse neural crest cell line. Here we describe detailed step-by-step protocols for culturing O9-1 cells, differentiating O9-1 cells into specific cell types, and genetically manipulating O9-1 cells by using siRNA-mediated knockdown or CRISPR-Cas9 genome editing.

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Cite this Article

Nguyen, B. H., Ishii, M., Maxson, R. E., Wang, J. Culturing and Manipulation of O9-1 Neural Crest Cells. J. Vis. Exp. (140), e58346, doi:10.3791/58346 (2018).

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