Journal
/
Immunology and Infection
/
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

English

Automatically Generated

8,609 Views

•

07:06 min

•

April 10, 2018

DOI:

10.3791/57371-v

DOI:
10.3791/57371-v

•

07:06 min
April 10, 2018

•

7 Views
,
1Department of Microbiology and Immunology,Uniformed Services University

Summary

Automatically generated

We describe a simple and easy protocol for measuring antibody dependent enhancement of infection by Zika virus convalescent serum using Dengue virus Reporter Viral Particles.

Explore More Videos

Flow CytometryAntibody Dependent EnhancementDengue VirusZika VirusConvalescent SerumRhesus MacaquesIn VitroVirus InfectionRPMISerial DilutionsDengue Virus SerotypesK562 CellsTrypan BlueHemocytometerCentrifugation

Read Article

Cite this Article

Valiant, W. G., Mattapallil, J. J. A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum. J. Vis. Exp. (134), e57371, doi:10.3791/57371 (2018).

Download .ris file

Copy

Share Video

.

Copy