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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
JoVE Journal
Genetics
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JoVE Journal Genetics
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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31,623 Views

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10:16 min

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June 28, 2018

DOI:

10.3791/57320-v

DOI:
10.3791/57320-v

•

10:16 min
June 28, 2018

•

12 Views
, , , ,
1Nuclear Dynamics Programme, The Babraham Institute,Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol,Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute,Babraham Research Campus, 5Department of Biological Science,Florida State University

Summary

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DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.

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Cite this Article

Schoenfelder, S., Javierre, B., Furlan-Magaril, M., Wingett, S. W., Fraser, P. Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions. J. Vis. Exp. (136), e57320, doi:10.3791/57320 (2018).

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