Journal
/
Immunology and Infection
/
Electrochemiluminescence Assays for Human Islet Autoantibodies
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Electrochemiluminescence Assays for Human Islet Autoantibodies

Electrochemiluminescence Assays for Human Islet Autoantibodies

English

Automatically Generated

15,020 Views

•

09:15 min

•

March 23, 2018

DOI:

10.3791/57227-v

DOI:
10.3791/57227-v

•

09:15 min
March 23, 2018

•

8 Views
, , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Endocrinology,First Affiliated Hospital of Nanjing Medical University

Summary

Automatically generated

Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune diseases.

Explore More Videos

ElectrochemiluminescenceIslet AutoantibodiesType 1 DiabetesAutoimmuneNon-radioactiveRadioassayELISAInsulin AutoantibodyBiotinSULFO-TAGSpin ColumnCheckerboard AssayPhosphate Buffered Saline

Read Article

Cite this Article

Gu, Y., Zhao, Z., Miao, D., High, H., Yang, T., Yu, L. Electrochemiluminescence Assays for Human Islet Autoantibodies. J. Vis. Exp. (133), e57227, doi:10.3791/57227 (2018).

Download .ris file

Copy

Share Video

.

Copy