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Electrochemiluminescence Assays for Human Islet Autoantibodies
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Electrochemiluminescence Assays for Human Islet Autoantibodies

Electrochemiluminescence Assays for Human Islet Autoantibodies

15,061 Views

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09:15 min

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March 23, 2018

DOI:

10.3791/57227-v

DOI:
10.3791/57227-v

•

09:15 min
March 23, 2018

•

8 Views
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1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Endocrinology,First Affiliated Hospital of Nanjing Medical University

Summary

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Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune diseases.

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ElectrochemiluminescenceIslet AutoantibodiesType 1 DiabetesAutoimmuneNon-radioactiveRadioassayELISAInsulin AutoantibodyBiotinSULFO-TAGSpin ColumnCheckerboard AssayPhosphate Buffered Saline

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Cite this Article

Gu, Y., Zhao, Z., Miao, D., High, H., Yang, T., Yu, L. Electrochemiluminescence Assays for Human Islet Autoantibodies. J. Vis. Exp. (133), e57227, doi:10.3791/57227 (2018).

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