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A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

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7,045 Views

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10:36 min

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January 09, 2018

DOI:

10.3791/56785-v

DOI:
10.3791/56785-v

•

10:36 min
January 09, 2018

•

2 Views
, , , , , , , , ,
1Department of Anatomical and Cellular Pathology,The Chinese University of Hong Kong, 2Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics,The Chinese University of Hong Kong

Summary

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Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in vivo.

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Tang, P. M., Tang, P. C., Chung, J. Y., Hung, J. S. C., Wang, Q., Lian, G., Sheng, J., Huang, X., To, K., Lan, H. A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro. J. Vis. Exp. (131), e56785, doi:10.3791/56785 (2018).

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