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Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
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December 08, 2017
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Recombinant prototype foamy virus integrase protein is often contaminated with a bacterial nuclease during purification. This method identifies nuclease contamination and removes it from the final preparation of the enzyme.
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Lopez Jr., M. A., Mackler, R. M., Altman, M. P., Yoder, K. E. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (130), e56605, doi:10.3791/56605 (2017).
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