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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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10,176 Views

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13:14 min

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February 06, 2018

DOI:

10.3791/56508-v

DOI:
10.3791/56508-v

•

13:14 min
February 06, 2018

•

5 Views
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1CRCINA, Inserm, CNRS, Université d’Angers,Université de Nantes, 2LabEx IGO, “Immunotherapy, Graft, Oncology”, 3CHU Nantes, France

Summary

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We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.

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Cite this Article

Devilder, M., Moyon, M., Saulquin, X., Gautreau-Rolland, L. Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood. J. Vis. Exp. (132), e56508, doi:10.3791/56508 (2018).

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