Journal
/
免疫与感染
/
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

10,176 Views

•

13:14 min

•

February 06, 2018

DOI:

10.3791/56508-v

DOI:
10.3791/56508-v

•

13:14 min
February 06, 2018

•

5 Views
, , ,
1CRCINA, Inserm, CNRS, Université d’Angers,Université de Nantes, 2LabEx IGO, “Immunotherapy, Graft, Oncology”, 3CHU Nantes, France

Summary

Automatically generated

We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.

Explore More Videos

Human Monoclonal AntibodiesAntigen-specific B CellsBloodDiscriminative AntibodiesImmunotherapyB LymphocytesDensity GradientMononuclear CellsTetramersMagnetic MicrobeadsCell Separation

Read Article

Cite this Article

Devilder, M., Moyon, M., Saulquin, X., Gautreau-Rolland, L. Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood. J. Vis. Exp. (132), e56508, doi:10.3791/56508 (2018).

Download .ris file

Copy

Share Video

.

Copy