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Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
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JoVE Journal Neuroscience
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

English

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7,062 Views

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11:12 min

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October 04, 2017

DOI:

10.3791/56425-v

DOI:
10.3791/56425-v

•

11:12 min
October 04, 2017

•

3 Views
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1Automation & Logistics Management, Screening Sciences & Novel Assay Technologies,Institut Pasteur Korea, 2School of Pharmacy,Sungkyunkwan University, 3Technology Development Platform,Institut Pasteur Korea

Summary

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We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.

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Lee, H., Radu, C., Han, J. W., Grailhe, R. Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells. J. Vis. Exp. (128), e56425, doi:10.3791/56425 (2017).

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