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Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
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Neuroscience
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JoVE Journal Neuroscience
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

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9,448 Views

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06:27 min

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October 27, 2017

DOI:

10.3791/56249-v

DOI:
10.3791/56249-v

•

06:27 min
October 27, 2017

•

7 Views
, , , ,
1Department of Ophthalmology, University Hospital of Bern,University of Bern, 2Department of Clinical Research,University of Bern, 3Graduate School for Cellular and Biomedical Sciences,University of Bern

Summary

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The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

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Cite this Article

Conedera, F. M., Arendt, P., Trepp, C., Tschopp, M., Enzmann, V. Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish. J. Vis. Exp. (128), e56249, doi:10.3791/56249 (2017).

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