Journal
/
神经科学
/
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

9,441 Views

•

06:27 min

•

October 27, 2017

DOI:

10.3791/56249-v

DOI:
10.3791/56249-v

•

06:27 min
October 27, 2017

•

7 Views
, , , ,
1Department of Ophthalmology, University Hospital of Bern,University of Bern, 2Department of Clinical Research,University of Bern, 3Graduate School for Cellular and Biomedical Sciences,University of Bern

Summary

Automatically generated

The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

Explore More Videos

Müller GliaRetinal DegenerationRetinal RegenerationLaser-induced InjuryZebrafishIn Vivo ImagingOptical Coherence Tomography (OCT)AnesthesiaTricaineHydroxypropyl MethylcelluloseFundus LaserHydrogel Contact Lens

Read Article

Cite this Article

Conedera, F. M., Arendt, P., Trepp, C., Tschopp, M., Enzmann, V. Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish. J. Vis. Exp. (128), e56249, doi:10.3791/56249 (2017).

Download .ris file

Copy

Share Video

.

Copy