/
/
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,739 Views
•
10:40 min
•
February 13, 2017
DOI:
Summary
Automatically generated
The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.
Explore More Videos
Ex VivoColon Organ CultureAntimicrobial Host DefensePathogen Associated Molecular PatternsPattern Recognition ReceptorsInnate Immune PathwaysAntimicrobial PeptidesIntestinal ArchitectureEuthanasiaPeritoneumCecumRectumPBSDMEM F12FBSPenicillin StreptomycinGentamycinCell Strainer12-well Cell Culture Plate
Read Article
Cite this Article
Udden, S. M. N., Waliullah, S., Harris, M., Zaki, H. The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies. J. Vis. Exp. (120), e55347, doi:10.3791/55347 (2017).
Copy