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Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
JoVE Journal
Genetics
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JoVE Journal Genetics
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

English

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9,930 Views

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07:40 min

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October 29, 2016

DOI:

10.3791/54752-v

DOI:
10.3791/54752-v

•

07:40 min
October 29, 2016

•

3 Views
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1Terrestrial Biofilms Group, Institute of Microbiology,Friedrich Schiller University, Jena

Summary

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The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis.

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Hölscher, T., Dragoš, A., Gallegos-Monterrosa, R., Martin, M., Mhatre, E., Richter, A., Kovács, Á. T. Monitoring Spatial Segregation in Surface Colonizing Microbial Populations. J. Vis. Exp. (116), e54752, doi:10.3791/54752 (2016).

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