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Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
JoVE Revista
Genética
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JoVE Revista Genética
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

Monitoring Spatial Segregation in Surface Colonizing Microbial Populations

DOI:
10.3791/54752-v

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07:40 min

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October 29, 2016

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, , , , , ,
1Terrestrial Biofilms Group, Institute of Microbiology,Friedrich Schiller University, Jena

Capítulos

  • 00:05Título
  • 00:47Preparation of Media and Starter Cultures
  • 01:54Co-inoculation of Fluorescently Labeled Strains for Surface Spreading
  • 03:16Co-inoculation of Fluorescently Labeled Strains for Biofilm Growth
  • 04:32Colony Imaging Using Fluorescent Microscopy
  • 05:36Results: Swarming, Sliding, and Biofilm Producing Colonies Display Different Patternsof Spatial Growth
  • 07:12Conclusion

Summary

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Traducción Automática

The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis.

Tags

Spatial SegregationMicrobial PopulationsFluorescent MicroscopySwarmingSlidingBiofilm FormationSocial MicrobiologyFitnessImage AnalysisB. SubtilisLB MediumSwarming PlatesSliding PlatesColony BiofilmSG MediumGreen And Red Fluorescent ProteinOptical DensityMicroscopic TechniquesMicrobial Interaction

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