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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
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07:27 min
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November 21, 2016
DOI:
1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital,Wenzhou Medical University, 2Departments of Urology,Boston Children’s Hospital, 3Department of Surgery,Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute,Guangdong General Hospital, Guangdong Academy of Medical Sciences
Summary
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We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
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Zhang, H., Huang, J., Liu, X., Zhu, P., Li, Z., Li, X. Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy. J. Vis. Exp. (117), e54625, doi:10.3791/54625 (2016).
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