Journal
/
Immunology and Infection
/
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

English

Automatically Generated

8,139 Views

•

15:29 min

•

August 05, 2016

DOI:

10.3791/54263-v

DOI:
10.3791/54263-v

•

15:29 min
August 05, 2016

•

1 Views
, , , , , ,
1Focal Area Infection Biology, Biozentrum,University of Basel, 2Centre d’Immunologie de Marseille-Luminy,Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical,Universidad de Navarra, 4BioDataAnalysis GmbH

Summary

Automatically generated

Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.

Explore More Videos

MicroscopyHigh-throughput ScreeningBrucella InfectionHeLa CellsHost FactorsSiRNACell CultureTransfectionAutomated WorkflowImage Analysis

Read Article

Cite this Article

Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).

Download .ris file

Copy

Share Video

.

Copy