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Immunology and Infection
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Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

English

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22,079 Views

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11:11 min

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February 08, 2016

DOI:

10.3791/53389-v

DOI:
10.3791/53389-v

•

11:11 min
February 08, 2016

•

3 Views
, , , , ,
1Institut für Anatomie und Zellbiologie,Justus-Liebig-Universität Gießen, 2Institut für Zytobiologie und Zytopathologie,Philipps-Universität Marburg

Summary

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Primary Sertoli cells are required for studying testis-immune privilege, signal transduction during inflammation or infection, and utilization of their immunoprotective properties. Here we describe an enzyme-based protocol for the isolation of highly purified primary Sertoli cells and peritubular cells from rat testes.

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Cite this Article

Bhushan, S., Aslani, F., Zhang, Z., Sebastian, T., Elsässer, H., Klug, J. Isolation of Sertoli Cells and Peritubular Cells from Rat Testes. J. Vis. Exp. (108), e53389, doi:10.3791/53389 (2016).

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