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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
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JoVE Journal Chemistry
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

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13,253 Views

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09:55 min

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July 04, 2016

DOI:

10.3791/53245-v

DOI:
10.3791/53245-v

•

09:55 min
July 04, 2016

•

2 Views
, , , ,
1Department of Biological Sciences, Markey Center for Structural Biology,Purdue University, 2National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK),National Institutes of Health, 3National Institute of General Medical Sciences (NIGMS),National Institutes of Health

Summary

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β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR spectroscopy.

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Cite this Article

Noinaj, N., Mayclin, S., Stanley, A. M., Jao, C. C., Buchanan, S. K. From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins. J. Vis. Exp. (113), e53245, doi:10.3791/53245 (2016).

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