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Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
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Neuroscience
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JoVE Journal Neuroscience
Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

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13,299 Views

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10:13 min

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August 12, 2014

DOI:

10.3791/51763-v

DOI:
10.3791/51763-v

•

10:13 min
August 12, 2014

•

2 Views
, , , , , , , ,
1Department of Pathology and Laboratory Medicine,University of North Carolina School of Medicine, 2Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine, 3Division of Neuropathology, Department of Pathology and Laboratory Medicine,University of North Carolina School of Medicine, 4Curriculum in Genetics and Molecular Biology,University of North Carolina School of Medicine, 5Biological and Biomedical Sciences Program,University of North Carolina School of Medicine, 6Department of Radiation Oncology,Emory University School of Medicine, 7Department of Neurology, Neurosciences Center,University of North Carolina School of Medicine

Summary

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Phenotypically wild-type astrocytes and neural stem cells harvested from mice engineered with floxed, conditional oncogenic alleles and transformed via viral Cre-mediated recombination can be used to model astrocytoma pathogenesis in vitro and in vivo by orthotopic injection of transformed cells into brains of syngeneic, immune-competent littermates.

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McNeill, R. S., Schmid, R. S., Bash, R. E., Vitucci, M., White, K. K., Werneke, A. M., Constance, B. H., Huff, B., Miller, C. R. Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice. J. Vis. Exp. (90), e51763, doi:10.3791/51763 (2014).

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