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Neuroscience

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JoVE Journal Neuroscience
Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
 

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Article DOI: 10.3791/51763-v • 10:13 min • August 12th, 2014
August 12th, 2014 •
1, 2, 3, 4, 1, 3, 5, 6, 2,3,7
1Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, 2Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, 3Division of Neuropathology, Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, 4Curriculum in Genetics and Molecular Biology, University of North Carolina School of Medicine, 5Biological and Biomedical Sciences Program, University of North Carolina School of Medicine, 6Department of Radiation Oncology, Emory University School of Medicine, 7Department of Neurology, Neurosciences Center, University of North Carolina School of Medicine

Kapitel

Summary
Automatic Translation

Phenotypically wild-type astrocytes and neural stem cells harvested from mice engineered with floxed, conditional oncogenic alleles and transformed via viral Cre-mediated recombination can be used to model astrocytoma pathogenesis in vitro and in vivo by orthotopic injection of transformed cells into brains of syngeneic, immune-competent littermates.

Tags

Astrocytoma Pathogenesis In Vitro In Vivo Cortical Astrocytes Neural Stem Cells Conditional Genetically Engineered Mice Oncogenic Mutations Brain Cell Types Preclinical Drug Development Model System Floxed Oncogenic Alleles Genetic Recombination Adenoviral Cre-mediated Recombination Tumor Suppressor Genes Proliferation Transformation Drug Response Orthotopic Allograft Models Tumorigenesis Immune-competent Syngeneic Littermates Glioblastoma Cell Line Xenografts
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