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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
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Biology
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Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

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7,578 Views

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12:43 min

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December 06, 2013

DOI:

10.3791/50856-v

DOI:
10.3791/50856-v

•

12:43 min
December 06, 2013

•

1 Views
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1Materials Science and Engineering,CSIRO

Summary

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We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population.

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Polanco, J. C., Wang, B., Zhou, Q., Chy, H., O'Brien, C., Laslett, A. L. Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2. J. Vis. Exp. (82), e50856, doi:10.3791/50856 (2013).

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