This content is Free Access.
Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
7,526 Views
•
12:43 min
•
December 06, 2013
DOI:
概要
Automatically generated
We describe the use of the monoclonal antibodies TG30 (CD9) and GCTM-2 for the combined detection of cell surface antigens via fluorescence activated cell sorting (FACS) for the identification and enrichment of live human embryonic stem cells (hESC) using positive selection and also the use of negative selection to purge hESCs from a mixed cell population.
Read Article
Cite this Article
Polanco, J. C., Wang, B., Zhou, Q., Chy, H., O'Brien, C., Laslett, A. L. Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2. J. Vis. Exp. (82), e50856, doi:10.3791/50856 (2013).
Copy