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Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

English

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13,717 Views

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08:09 min

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May 18, 2014

DOI:

10.3791/50796-v

DOI:
10.3791/50796-v

•

08:09 min
May 18, 2014

•

4 Views
, , ,
1Pediatric Diabetes Research Center, Department of Pediatrics,University of California, San Diego

Summary

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A protocol to isolate, culture, and image islet cell clusters (ICCs) derived from human fetal pancreatic cells is described.  The method details the steps necessary to generate ICCs from tissue, culture as monolayers or in suspension as aggregates, and image for markers of proliferation and pancreatic cell fate decisions.

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Lopez, A. D., Kayali, A. G., Hayek, A., King, C. C. Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters. J. Vis. Exp. (87), e50796, doi:10.3791/50796 (2014).

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