This content is Free Access.
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
13,717 Views
•
08:09 min
•
May 18, 2014
DOI:
Summary
Automatically generated
A protocol to isolate, culture, and image islet cell clusters (ICCs) derived from human fetal pancreatic cells is described. The method details the steps necessary to generate ICCs from tissue, culture as monolayers or in suspension as aggregates, and image for markers of proliferation and pancreatic cell fate decisions.
Read Article
Cite this Article
Lopez, A. D., Kayali, A. G., Hayek, A., King, C. C. Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters. J. Vis. Exp. (87), e50796, doi:10.3791/50796 (2014).
Copy