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A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

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14,399 Views

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16:04 min

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June 19, 2013

DOI:

10.3791/50606-v

DOI:
10.3791/50606-v

•

16:04 min
June 19, 2013

•

2 Views
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1Mork Family Department of Chemical Engineering and Materials Science, Viterbi School of Engineering,University of Southern California, Los Angeles

Summary

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Here, we use retroviral transduction and concatemeric transfection to create a cell line that can express the components of a lentiviral vector (LV) in the absence of tetracycline. This LV encodes GFP and is pseudotyped with a glycoprotein, SVGmu, which is specific for a receptor on dendritic cells.

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Lentiviral VectorsHigh-titerDendritic CellsTet-offProducer Cell LineConcatemeric DNA TransfectionReporter GeneTransductionT Cell Immune Response

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Cite this Article

Bryson, P. D., Zhang, C., Lee, C., Wang, P. A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells. J. Vis. Exp. (76), e50606, doi:10.3791/50606 (2013).

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