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A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
JoVE Journal
Medicine
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JoVE Journal Medicine
A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

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11,242 Views

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12:09 min

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June 16, 2013

DOI:

10.3791/50363-v

DOI:
10.3791/50363-v

•

12:09 min
June 16, 2013

•

1 Views
, , , , ,
1Brain Tumor Research Centre,Hospital for Sick Children, Toronto Medical Discovery Tower, 2Ontario Cancer Institute,Princess Margaret Hospital, 3Neurosurgery,Toronto Western Hospital

Summary

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We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.

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Cite this Article

Burrell, K., Agnihotri, S., Leung, M., DaCosta, R., Hill, R., Zadeh, G. A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics. J. Vis. Exp. (76), e50363, doi:10.3791/50363 (2013).

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