/
/
Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development
A subscription to JoVE is required to view this content. Sign in or start your free trial.
12,752 Views
•
13:47 min
•
April 03, 2013
DOI:
Summary
Automatically generated
This protocol describes an improved explant procedure that involves ex utero electroporation, dissection and culture of entire cerebral hemispheres from the embryonic mouse. The preparation facilitates pharmacological studies and assays of gene function during early cortical development.
Read Article
Cite this Article
Nichols, A. J., O'Dell, R. S., Powrozek, T. A., Olson, E. C. Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development. J. Vis. Exp. (74), e50271, doi:10.3791/50271 (2013).
Copy