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Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
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November 26, 2012
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The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.
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Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation
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Pannasch, U., Sibille, J., Rouach, N. Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices. J. Vis. Exp. (69), e4418, doi:10.3791/4418 (2012).
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