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Preparation of Drosophila Central Neurons for in situ Patch Clamping
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparation of Drosophila Central Neurons for in situ Patch Clamping

Preparation of Drosophila Central Neurons for in situ Patch Clamping

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08:27 min

October 15, 2012

DOI:

08:27 min
October 15, 2012

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Summary

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In situ patch clamp recordings are used for electrophysiological characterization of neurons in intact circuitry. In the Drosophila genetic model patch clamping is difficult because the CNS is small and surrounded by a robust sheath. This article describes the procedure to remove the sheath and clean neurons for subsequent patch clamp recordings.

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