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Preparation of Drosophila Central Neurons for in situ Patch Clamping
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Preparation of Drosophila Central Neurons for in situ Patch Clamping
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October 15, 2012
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In situ patch clamp recordings are used for electrophysiological characterization of neurons in intact circuitry. In the Drosophila genetic model patch clamping is difficult because the CNS is small and surrounded by a robust sheath. This article describes the procedure to remove the sheath and clean neurons for subsequent patch clamp recordings.
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Cite this Article
Ryglewski, S., Duch, C. Preparation of Drosophila Central Neurons for in situ Patch Clamping. J. Vis. Exp. (68), e4264, doi:10.3791/4264 (2012).
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