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May 24, 2012
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We describe a rapid methodology to isolate and culture hippocampal and cortical neurons from rodent embryos. This protocol allows us to perform experiments in which nearly pure neuronal cultures are required.
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IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection
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Pacifici, M., Peruzzi, F. Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol. J. Vis. Exp. (63), e3965, doi:10.3791/3965 (2012).
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