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October 21, 2011
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A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
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Neural Tube ClosureMouse EmbryosEmbryo CultureGenetic Mouse ModelsMammalian Neural Tube ClosureMaternal MetabolismPharmacological ManipulationSmall Molecule DeliveryRecombinant Protein DeliverySiRNA DeliveryWhole Embryo CultureEnriched MediaRoller Incubator ApparatusNormal Neural Tube ClosureIn Vitro TechniquesNeurulationNeural Plate FormationCranial Fold ClosureCaudal Neuropore ClosureEmbryo Dissection
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Gray, J., Ross, M. E. Neural Tube Closure in Mouse Whole Embryo Culture. J. Vis. Exp. (56), e3132, doi:10.3791/3132 (2011).
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