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April 25, 2011
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In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.
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Transcriptome AnalysisSingle CellsGene Expression AnalysisHeterogeneous PopulationsCell CultureHippocampusMRNA ProfilesMolecular DifferencesCell PopulationsHarvesting CellsElectrophysiologyEppendorf TubeMolecular Biology TechniquesARNA AmplificationDownstream ApplicationReverse-transcriptionReal-time Polymerase Chain Reaction (PCR)Linear AmplificationPolyadenylated RNA
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Morris, J., Singh, J. M., Eberwine, J. H. Transcriptome Analysis of Single Cells. J. Vis. Exp. (50), e2634, doi:10.3791/2634 (2011).
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