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February 22, 2011
DOI:
1Cardiovascular Research Institute,University of California San Francisco, 2Department of Pediatrics,University of California San Francisco, 3Department of Biology,San Francisco State University, 4Department of Medicine,University of California San Francisco , 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research,University of California San Francisco
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To assess the in vivo effects of therapeutic interventions for muscle disease, methods are needed to quantitate force generation and fatigability in treated muscle. We detail an approach to evaluating myo-mechanical properties in explanted mouse hindlimb muscle. This analysis provides a robust approach to quantitating the effects of genetic modification on muscle function, as well as comparison of therapies in mouse models of muscle disease.
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Myo-mechanical AnalysisIsolated Skeletal MuscleTherapeutic InterventionsMuscle DiseaseQuantitative MethodsForce GenerationFatigabilityHindlimb MuscleExplanted MuscleMuscle Strip MyographMaximal Twitch TensionTetanic TensionContraction TimeHalf-relaxation TimeSquare Pulse StimulatorSpecific Twitch TensionSpecific Tetanic TensionMuscle Cross-sectional AreaTwitch-to-tetanic Tension RatioForce-frequency Relationship CurveLow Frequency Fatigue CurveComparison Between Therapeutic InterventionsMouse Models Of Muscle DiseaseGenetic ModificationMuscle Function
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Oishi, P. E., Cholsiripunlert, S., Gong, W., Baker, A. J., Bernstein, H. S. Myo-mechanical Analysis of Isolated Skeletal Muscle. J. Vis. Exp. (48), e2582, doi:10.3791/2582 (2011).
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