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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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27,187 Views

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09:45 min

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February 04, 2011

DOI:

10.3791/2545-v

DOI:
10.3791/2545-v

•

09:45 min
February 04, 2011

•

3 Views
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1Electrical and Computer Engineering,University of Illinois at Urbana-Champaign, 2Micro and Nanotechnology Lab,University of Illinois at Urbana-Champaign, 3Mechanical Science and Engineering,University of Illinois at Urbana-Champaign, 4Bioengineering,University of Illinois at Urbana-Champaign

Summary

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Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.

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Microfluidic DevicesCell StudiesFluidic EnvironmentManipulating CellsSorting CellsCounting CellsDielectrophoresisLaminar FlowPrinted Circuit Boards (PCBs)Non-contact ManipulationBead ManipulationCell ManipulationBioactuationCell SeparationMultichannel Microfluidic DevicesDEP Electrodes

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Millet, L. J., Park, K., Watkins, N. N., Hsia, K. J., Bashir, R. Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow. J. Vis. Exp. (48), e2545, doi:10.3791/2545 (2011).

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