Rivista
/
Bioingegneria
/
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

27,185 Views

•

09:45 min

•

February 04, 2011

DOI:

10.3791/2545-v

DOI:
10.3791/2545-v

•

09:45 min
February 04, 2011

•

3 Views
, , , ,
1Electrical and Computer Engineering,University of Illinois at Urbana-Champaign, 2Micro and Nanotechnology Lab,University of Illinois at Urbana-Champaign, 3Mechanical Science and Engineering,University of Illinois at Urbana-Champaign, 4Bioingegneria,University of Illinois at Urbana-Champaign

Summary

Automatically generated

Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.

Explore More Videos

Microfluidic DevicesCell StudiesFluidic EnvironmentManipulating CellsSorting CellsCounting CellsDielectrophoresisLaminar FlowPrinted Circuit Boards (PCBs)Non-contact ManipulationBead ManipulationCell ManipulationBioactuationCell SeparationMultichannel Microfluidic DevicesDEP Electrodes

Read Article

Cite this Article

Millet, L. J., Park, K., Watkins, N. N., Hsia, K. J., Bashir, R. Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow. J. Vis. Exp. (48), e2545, doi:10.3791/2545 (2011).

Download .ris file

Copy

Share Video

.

Copy