/
/
Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
A subscription to JoVE is required to view this content. Sign in or start your free trial.
14,675 Views
•
09:14 min
•
March 17, 2011
DOI:
Summary
Automatically generated
A method to visualize and quantify F-actin barbed ends in neuronal growth cones is described. After culturing neurons on glass coverslips, cells are permeabilized with a saponin-containing solution. Then, a short incubation with the saponin buffer containing rhodamine-actin incorporates fluorescent actin onto free actin barbed ends.
Explore More Videos
F-actinBarbed EndsRhodamine-actinPermeabilizedNeuronal Growth ConesAxonsMolecular Guidance CuesGrowth Cone CytoskeletonActin Filament MeshworkActin PolymerizationDynamic RemodelingGuidance CuesActin MonomerPolymerization NucleiChick RetinalDorsal Root Ganglion (DRG)Actin Depolymerizing Factor (ADF/cofilin)
Read Article
Cite this Article
Marsick, B. M., Letourneau, P. C. Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones. J. Vis. Exp. (49), e2409, doi:10.3791/2409 (2011).
Copy