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Generating a Coronal Brain Slice Exposing the Amygdala Regions from a Mouse Brain
Transcript
After obtaining the brain, use a scalpel to remove the cerebellum and interior part of the brain, and then cool the middle portion of the brain in ice-cold cutting solution as before. Blot the brain with filter paper, and then glue it to the vibratome stage.
Again, fix an additional agar block behind the brain for stability while slicing. After fitting the stage into the cutting chamber filled with 4 degrees Celsius cutting solution, cut 320-micron coronal sections through the amygdala. Cut them in half and collect the sections in the interface chamber as before.
