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October 16, 2010
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Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.
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Mammary Gland MorphologyBreast Cancer RiskRatsDietary FactorsHormonal FactorsIn UteroPubertal PeriodsModeling And Re-modelingSusceptibility To Carcinogen-induced TumorsHigh BirthweightSoy IntakePrenatal And Postnatal Dietary ModificationsPersistent Morphological ChangesEpigenetic ModificationsDNA MethylationHistonesMiRNA ExpressionGene TranscriptionDissecting And Removing Abdominal Mammary GlandPreparing Mammary Gland Whole MountsAnalyzing Mammary Gland MorphologyTerminal End BudsEpithelial Elongation And DifferentiationPredicting Risk Of Developing Mammary Cancer
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de Assis, S., Warri, A., Cruz, M. I., Hilakivi-Clarke, L. Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats. J. Vis. Exp. (44), e2260, doi:10.3791/2260 (2010).
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