Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model
Transcript
Begin with an anesthetized mouse under a stereomicroscope, with the underside of one of the mouse's ears fixed on the stage via adhesive tape.
The eyes are coated with an ointment to prevent them from drying.
Bring a syringe loaded with the sporozoite stage of Plasmodium, a malarial parasite. The live sporozoites are radiation-attenuated to render them non-infectious.
Using the stereomicroscope, inject into the top side of the ear, penetrating the outermost epidermis layer and releasing the sporozoites into the dermis.
Keep the needle in place briefly to prevent backflow of the injected suspension before removing it.
A visible swelling at the injection site, termed a papule, confirms a successful injection.
Perform multiple injections into the same ear.
Release the injected ear and repeat the injections into the other ear.
Post-injection, allow the mouse to recover.
The injected sporozoites trigger an immune response, priming the immune system against the parasite.
