A subscription to JoVE is required to view this content.  Sign in or start your free trial.
Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia

Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia

Transcript

Immediately after retrieval, use a standard 14-gauge peripheral catheter to cannulate the renal artery, and flush the kidney with at least 500 milliliters of ice-cold organ preservation solution. After the flush, wrap the graft in sterile organ bags for storage in organ preservation solution, with a target cold ischemic time of 24 hours at 4 degrees Celsius using a computer-controlled cooling circuit.

Related Videos

Read Article