Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia

Immediately after retrieval, use a standard 14-gauge peripheral catheter to cannulate the renal artery, and flush the kidney with at least 500 milliliters of ice-cold organ preservation solution. After the flush, wrap the graft in sterile organ bags for storage in organ preservation solution, with a target cold ischemic time of 24 hours at 4 degrees Celsius using a computer-controlled cooling circuit.