Journal
/
Immunology and Infection
/
Generation of Recombinant Influenza Virus from Plasmid DNA
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Recombinant Influenza Virus from Plasmid DNA

English

Automatically Generated

29,857 Views

•

11:31 min

•

August 03, 2010

DOI:

10.3791/2057-v

DOI:
10.3791/2057-v

•

11:31 min
August 03, 2010

•

29 Views
,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Summary

Automatically generated

Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.

Explore More Videos

Recombinant Influenza VirusPlasmid DNAReverse GeneticsInfluenza ResearchInfectious VirusesViral ProteinsMutationsViral ReplicationPathogenesisViral VectorsForeign Proteins

Read Article

Cite this Article

Martínez-Sobrido, L., García-Sastre, A. Generation of Recombinant Influenza Virus from Plasmid DNA. J. Vis. Exp. (42), e2057, doi:10.3791/2057 (2010).

Download .ris file

Copy

Share Video

.

Copy