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January 06, 2010
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Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
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FabricationOperationOxygen InsertAdherent Cellular CulturesOxygen ModulationBiomedical ResearchHypoxic ChamberTemporal ControlSpatial ControlPhysiological PhenomenaSpecialized KnowledgeEquipmentMicrofabricated InsertMultiwell PlatesPolydimethylsiloxane InsertPassive Microfluidic Gas NetworkGas-permeable MembraneOxygen DeliveryGas CylindersFabrication TechniquesSU-8 PhotolithographyReplica MoldingPDMS SpinningSilicon Wafer
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Oppegard, S., Sinkala, E., Eddington, D. Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures . J. Vis. Exp. (35), e1695, doi:10.3791/1695 (2010).
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