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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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生物学
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JoVE Journal 生物学
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

DOI:
10.3791/1695-v

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11:56 min

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January 06, 2010

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, ,
1生物工学,University of Illinois

章

  • 00:00標題
  • 00:49Introduction
  • 01:21Material Fabrication and Assembly of the Oxygenating Insert
  • 03:57Validation of Oxygenating Insert- Calibration, Heterogeneity and Equilibration.
  • 07:45Assembly of Oxygenating Insert for Wound Healing Cell Culture
  • 09:56Validating and Using an Oxygenating Insert in Monolayer Wound Healing
  • 10:59Conclusion

概要

自動翻訳

自動翻訳

Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.

タグ

FabricationOperationOxygen InsertAdherent Cellular CulturesOxygen ModulationBiomedical ResearchHypoxic ChamberTemporal ControlSpatial ControlPhysiological PhenomenaSpecialized KnowledgeEquipmentMicrofabricated InsertMultiwell PlatesPolydimethylsiloxane InsertPassive Microfluidic Gas NetworkGas-permeable MembraneOxygen DeliveryGas CylindersFabrication TechniquesSU-8 PhotolithographyReplica MoldingPDMS SpinningSilicon Wafer

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