Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Melanie Jungblut; Karen Oeltze; Irene Zehnter; Doris Hasselmann; Andreas Bosio

doi:10.3791/1029

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Biology

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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

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Biology

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JoVE Journal Biology

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

37,225 Views

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05:18 min

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December 11, 2008

DOI:

10.3791/1029-v

DOI:

10.3791/1029-v

•

05:18 min

December 11, 2008

•

30 Views

Melanie Jungblut¹, Karen Oeltze¹, Irene Zehnter¹, Doris Hasselmann¹, Andreas Bosio¹

¹Miltenyi Biotec,GmbH

Summary

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

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