JOVE JOURNAL

Biochemistry

Showing 685 - 696 in 776 videos.

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Cristina Banfi¹, Anna Guarino², Maura Brioschi¹, Stefania Ghilardi¹, Valeria Mastrullo¹, Elena Tremoli¹, Gianluca Polvani^2,3,4

¹Centro Cardiologico Monzino IRCCS, ²Cardiovascular Tissue Bank of Milan,Centro Cardiologico Monzino IRCCS, ³Department of Clinical Sciences and Community Health, Cardiovascular Section,University of Milan, ⁴Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit,Centro Cardiologico Monzino IRCCS

Journal (Biochemistry)

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Xun Zhang¹, Sheng Chen¹, Feng Xu¹

¹Beijing Key Laboratory of Lignocellulosic Chemistry,Beijing Forestry University

Journal (Biochemistry)

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Mahnoush Bahjat¹, Timon A. Bloedjes¹, Amélie van der Veen¹, Guus de Wilde¹, Chiel Maas¹, Jeroen E. J. Guikema¹

¹Department of Pathology,Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

Journal (Biochemistry)

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Chirag Vasavda*¹, Nicholas W. Zaccor*¹, Paul C. Scherer¹, Charlotte J. Sumner^1,2, Solomon H. Snyder^1,3,4

¹The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, ²Department of Neurology and neurosurgery,Johns Hopkins University School of Medicine, ³Departments of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, ⁴Department of Psychiatry and Behavioral Sciences,Johns Hopkins University School of Medicine

Journal (Biochemistry)

Real-time Tracking of DNA Fragment Separation by Smartphone

Chunxian Tao¹, Bo Yang¹, Zhenqing Li¹, Dawei Zhang¹, Yoshinori Yamaguchi^1,2,3

¹Shanghai Key Lab of Modern Optical System,University of Shanghai for Science and Technology, ²Department of Applied Physics, Graduate School of Engineering,Osaka University, ³East China University of Science and Technology,Department of Physics Faculty of Science

Journal (Biochemistry)

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Mohamed Salem^1,2, Michal Bernach¹, Krzysztof Bajdzienko¹, Patrick Giavalisco¹

¹Max Planck Institute of Molecular Plant Physiology, Golm, Germany, ²Department of Pharmacognosy, Faculty of Pharmacy,Cairo University

Journal (Biochemistry)

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution

Gisela Beutner¹, George Arthur Porter Jr.¹

¹Department of Pediatrics-Division Cardiology,University of Rochester

Journal (Biochemistry)

Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids

Tanja Kalstrup¹, Rikard Blunck^1,2

¹Department of Pharmacology and Physiology,University of Montréal, ²Department of Physics,University of Montréal

Journal (Biochemistry)

Assaying Protein Kinase Activity with Radiolabeled ATP

Aroon S. Karra¹, Steve Stippec¹, Melanie H. Cobb¹

¹Department of Pharmacology,University of Texas Southwestern Medical Center

Journal (Biochemistry)

Oligopeptide Competition Assay for Phosphorylation Site Determination

Min Sung Joo¹, Ja Hyun Koo¹, Sol-Bi Shin², Hyungshin Yim², Sang Geon Kim¹

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Journal (Biochemistry)

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Rachel A. Battaglia¹, Parijat Kabiraj¹, Helen H. Willcockson¹, Melinda Lian¹, Natasha T. Snider¹

¹Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Journal (Biochemistry)

Measuring Protein Binding to F-actin by Co-sedimentation

Jonathon A. Heier¹, Daniel J. Dickinson², Adam V. Kwiatkowski¹

¹Department of Cell Biology,University of Pittsburgh School of Medicine, ²Department of Biology,University of North Carolina at Chapel Hill

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